Abstract
Aim: Identifying genes and pathways involved in various stages of rotavirus infection could facilitate the mapping of the interactions between the virus and host. Materials & methods: Weighted gene co-expression network analysis (WGCNA) was employed to construct a co-expression network of genes identified by microarray analysis from samples from children with acute rotavirus infection. Results: Two specific modules were most related to rotavirus infection. The analysis of differentially expressed genes (DEGs) disclosed 393 DEGs between normal and infected individuals. Eighteen novel genes were shared between the modules. Conclusion: The WGCNA revealed novel modules, co-expressed genes and pathways involved in immune responses, cell death/degradation and the nervous system in acute rotavirus infection.
Plain language summary
Rotaviruses are a type of virus that can cause diarrhea and vomiting, especially in children under 5 years old. In some cases, rotavirus infection can cause neurological disorders like seizures, reduced consciousness and inflammation of the brain. There is no cure for rotavirus infection. Treatment involves drinking plenty of water and symptom relief. To improve our understanding of how rotavirus causes disease, we analyzed the genetic data of children infected with rotavirus and healthy children. We identified genes in infected children that may be linked to rotavirus infection and disease. This information can be useful for identifying new targets for antiviral treatments and vaccines.
Supplementary data
To view the supplementary data that accompany this paper please visit the journal website at:www.tandfonline.com/doi/full/10.2217/fvl-2023-0045
Author contributions
S Samadizadeh: conceptualization, writing and editing the manuscript; S Samadizadeh and A Vaez: investigation and formal analysis; A Tahamtan and Z Jamalpoor: supervision and revising of the manuscript. All authors read and approved the final manuscript.
Financial & competing interests disclosure
The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.
No writing assistance was utilized in the production of this manuscript.