Investigation of Adipogenic Effects of Human Adenovirus Serotypes 36 and 5 in a Colo-320 Cell Line

Abstract

Aim: We aimed to investigate the adipogenic effects of adenovirus (Ad) serotypes 5, 36 and 8 in a Colo-320 cell line using histochemical, immunohistochemical and electron microscopic methods. Materials & methods: Ad serotypes were inoculated in a Colo-320 cell line and were cultured for 14 days. They were then collected and fixed with 4% paraformaldehyde to analyze their adipogenic effects using histochemistry, immunohistochemistry and electron microscopic methods. Intracellular lipid droplets were detected in the Colo-320 cells inoculated with Ad36 and Ad5 by electron microscopic analyses. Results: After Oil Red O staining, the pink–orange staining was positive intracellularly in Colo-320 cells infected with Ad36 and Ad5. In addition, the leptin immunoreactivity was also positive in these cells. Conclusion: Our results suggested that intracellular lipid accumulation occured after infection with Ad36 and Ad5. The positive staining of Oil Red O and leptin also supported the electron microscopic results; therefore, we conclude that this accumulation occurred due to adipogenic effects of Ad36 and Ad5.

Keywords:

• adenovirus serotypes
• adipogenic effect
• cell line
• electron microscopy
• immunohistochemical method

Financial & competing interests disclosure

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.