EGCG/Gelatin-Doxorubicin Gold Nanoparticles Enhance Therapeutic efficacy of Doxorubicin for Prostate Cancer Treatment

Abstract

Aim: Development of epigallocatechin gallate (EGCG) and gelatin-doxorubicin conjugate (GLT-DOX)-coated gold nanoparticles (DOX-GLT/EGCG AuNPs) for fluorescence imaging and inhibition of prostate cancer cell growth. Materials & methods: AuNPs alternatively coated with EGCG and DOX-GLT conjugates were prepared by a layer-by-layer assembly method. The physicochemical properties of the AuNPs and the effect of Laminin 67R receptor-mediated endocytosis on the anticancer efficacy of the AuNPs were examined. Results: The AuNPs significantly inhibit the proliferation of PC-3 cancer cell and the enzyme-responsive intracellular release of DOX could be tracked by monitoring the recovery of the fluorescence signal of DOX. Conclusion: Laminin 67R receptor-mediated delivery of DOX using the AuNPs enhanced cellular uptake of DOX and improved apoptosis of PC-3 cells.

Keywords::

• cellular uptake
• doxorubicin
• drug release
• EGCG
• gelatin
• gold nanoparticles
• Lam 67R
• MMP-2
• prostate cancer

Acknowledgements

The authors thank AC Chun, CM Lee and HM Chen (Core Facility Center, Taipei Medical University) for technical support in CLSM 3-D imaging and TEM analysis.

Financial & competing interests disclosure

This work was supported by a grant (NTUT-TMU-103–13) from Taipei Medical University and National Taipei University of Technology, Taiwan. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutionalreview board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. Inaddition, for investigations involving human subjects, informed consent hasbeen obtained from the participants involved.

Additional information

Funding

This work was supported by a grant (NTUT-TMU-103–13) from Taipei Medical University and National Taipei University of Technology, Taiwan. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.