Negative Neuronal Differentiation of Human Adipose-Derived Stem Cell Clones

Abstract

Aims: Adipose mesenchymal stem cells are a heterogeneous population. Therefore, the question posed in this study is whether the heterogenic differentiation potential exhibited by the different clones toward mesodermic lineages can be extended to nonmesodermic lineages, such as the neuroectoderm. Materials & methods: Different single cell clones of human adipose mesenchymal stem cells from the same donor were isolated. Neuronal plasticity of the clones was assessed according to the pattern DNA methylation, gene expression and intracellular calcium responses. Results: Under neurogenic culture conditions, clones presented variable expression of neuronal-specific genes, but still expressed osteogenic markers. No calcium response was exhibited in response to KCl incubation. The DNA methylation profile presented a very similar pattern in neuroectoderm gene promoters. Conclusions: Data indicate that there are no significant differences between the undifferentiated and supposedly neuronal-differentiated mesenchymal cells.

Keywords::

• adipose tissue
• adult stem cells
• cell therapy
• neurodegenerative diseases
• neuron

Acknowledgements

The authors would like thank the Spanish ‘Centro Nacional de Genotipado’ (CEGEN-ISCIII; www.cegen.org) for providing SNP genotyping services.

Supplementary data

Financial & competing interests disclosure

E Roche is recipient of the following grants: Instituto de Salud Carlos III-FEDER (PS09/01093), Fundacion Salud 2000-Merck Serono, PROMETEO/2012/007 from Generalitat Valenciana and ‘Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición’ CIBERobn (CB12/03/30038). MI Arribas is recipient of a fellowship of the VALi+d Program from Generalitat Valenciana (APOSTD/2012/021). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate insti­tutional review board approval or have followed the princi­ples outlined in the Declaration of Helsinki for all human or animal experimental investigations.

Additional information

Funding

E Roche is recipient of the following grants: Instituto de Salud Carlos III-FEDER (PS09/01093), Fundacion Salud 2000-Merck Serono, PROMETEO/2012/007 from Generalitat Valenciana and ‘Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición’ CIBERobn (CB12/03/30038). MI Arribas is recipient of a fellowship of the VALi+d Program from Generalitat Valenciana (APOSTD/2012/021). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.