Genetic Identification of Soybean [Glycine Max (L.) Merr.] Growing in Turkey for Molecular Breeding using Molecular Markers

ABSTRACT

The Soybean (Glycine max) is a species of legume which is a plant in the family Fabaceae. It is an annual plant that has been used in China and in other countries for 5 000 years as a food and a component of drugs. Soy contains significant amounts of all the essential amino acids for humans, and therefore is a good source of protein. Soybeans are the primary ingredient in many processed foods, including dairy product substitutes. The feasibility of detecting yields quality in soybean genotypes by a polymerase chain reaction (PCR) method is determined. PCR is a sensitive method for analyzing DNA and it is considered the most important method for detection of soybeans in processed or raw foods. Namely, PCR is a commonly applied nucleic amplification method which is specific and sensitive enough to detect even tiny amounts of organism-specific DNA sequences. This study focuses on the PCR detection in food, describes rapid and reliable DNA extraction methods which can be applied to a variety of food samples and details of PCR amplification protocols for sensitive and specific detection of soybean genotypes growing in Turkey. All soybean samples were evidenced by all PCR primers as soybean products.

Keywords:

• genomic DNA extraction
• soybean (Glycine max. L.)
• fingerprinting
• molecular marker