Callus Induction and Plant Regeneration from In Vitro Cultured Leaves, Petioles and Scales of Lilium Leucanthum (Baker) Baker

ABSTRACT

Lilium leucanthum is a high-value ornamental and medicinal plant in China. The plant has low propagation rate in nature; therefore, identification of a suitable system for in vitro propagation is needed. The aim of current study was to identify a suitable system for optimum callus induction and plant regeneration from in vitro cultured leaves, petioles and scales of L. leucanthum. Bulblets regeneration was tested on MS medium supplemented with different concentrations of BA and NAA. The highest frequency of regeneration (96.7%) and the largest mean number of bulblets per scale (3.07) were obtained on MS medium containing 0.5 mg l⁻¹ BA and 0.1 mg l⁻¹ NAA. In vitro cultured scale explants showed great ability to induce callus, followed by in vitro cultured petioles and leaves. MS medium with 1.0 mg l⁻¹ BA and 1.0 mg l⁻¹ 2,4-D was found to be optimal for callus induction from in vitro leaves and petioles with the highest induction percentages of 79.6% and 83.3%, respectively. MS medium containing 0.5 mg l⁻¹ BA and 1.0–3.0 mg l⁻¹ 2,4-D was found best for callus induction from scales with the highest percentage (98.3%). The largest number of bulblets was regenerated (23.9) from 1.0 g of fresh callus cultured on MS supplemented with 0.5 mg l⁻¹ BA and 0.2 mg l⁻¹ NAA. The number of roots per plant was fewer and the length of roots was greater on the rooting media with AC compared to the media without AC; half-strength MS medium with 0.05 mg l⁻¹ NAA was more suitable for rooting. After acclimatization, transplanted plantlets grew normally under greenhouse conditions.

Keywords:

• Lilium leucanthum
• plant regeneration
• callus induction