Turku sugar studies VIII: Principal microbiological findings

Abstract

The possible qualitative and/or quantitative alterations in the proportions of cultivable groups of oral microorganisms were analysed during a clinical trial involving the consumption of fructose (F) or xylitol (X) in comparison to sucrose (S). Supragingival plaque samples and paraffin-stimulated saliva were collected from 115 subjects. The samples were dispersed by sonication, diluted stepwise, plated on blood agar, Mac Leod agar, MacConcey agar, Rogosa S.L. agar, and Sabouraud agar plates and incubated anaerobically and/or aerobically. The number of the total colony forming units (CFU) on blood agar plates in anaerobic incubation was about 1–3 × 10⁹/ml saliva and 1–4 × 10⁸/mg plaque and in aerobic respectively 5–18 × 10⁸/ml saliva and 10⁸/mg plaque. The total CFU on Mac Leod agar was of a similar order of magnitude. The variation between subjects and consecutive determinations was relatively large. The arithmetic mean of the total CFU on MacConcey agar was about 1–5 × 10⁵/ml saliva, on Rogosa S.L. agar 6–130 × 10³/ml saliva and on Sabouraud about 1–2 × 10³/ml saliva, all in aerobic incubations. Replacement of dietary sucrose with xylitol did not affect the proportion of major microbial categories in saliva or dental plaque. The percentage of typical streptococcal colonies on blood agar was of a similar order of magnitude (about 60–70 %) during the diets. The arithmetic and geometric means of the total CFU values on Rogosa and Sabouraud agar plates were significantly lower in the X-group than in the S- or F-groups after a diet period of some months. It was thought that the reason for the reduction of acidogenic and aciduric oral flora in the X-group was partly due to the fact that xylitol is generally not metabolized by these microorganisms.

Key Words:

• Microbiology
• saliva
• plaque
• sucrose
• fructose
• xylitol