Abstract
Dental plaque samples collected from the subjects during the last 20 months of the 2 year trial were subjected to quantitative and qualitative analysis of the occurrence of S. mutatis, S. sanguis, S. salivarius and the total growth on phenol red agar. Lyophilized plaque samples were homogenized and incubated on a sucrose containing medium under anaerobic conditions. In addition, the pH-values were measured after incubation of the mixed plaque flora in media containing 1 % respectively xylitol (X), sorbitol, sucrose (S), fructose (F) or no carbohydrates. The results show a significantly lower incidence of S. mutatis in the X-group relative to the S- and F-groups. The corresponding difference could not be observed between the S- and F-groups. The logarithmic means and standard deviations of the colony counts of S. sanguis, S. salivarius and total bacteria yielded no significant differences between the 3 sugar groups during the test period. Repeated pH-measurements, carried out at the 4, 12, 18 and 24 month phases, showed that, except in the presence of X, the mean values all fell below the pH-limit of 5.5. In the course of the study, no evidence was obtained of adaption or mutation enabling acidogenic decomposition of X. These findings emphasize the importance of low acidogenic potential in dental plaque, generally parallelled by a low incidence of dental caries.