Estrogen receptors in the inner ear during different stages of pregnancy and development in the rat

Abstract

Conclusion: ERα and ERβ are present in the inner ear and are up- and down-regulated depending on the stage of maturation, development and pregnancy, suggesting that estrogen may have an effect on the cochlea during various stages of life. No estrogen receptors (ERs) were found in the cochlea of the developing fetus, which suggests that estrogen does not have an effect on the cochlea during gestation. Objective: To investigate the distribution of ERs in the cochlea during pregnancy, maturation and development in a female rat model. Materials and methods: The cochleas of 24 rats in 4 groups in different time periods of maturation (21 and 56 days old) and pregnancy (day 8 and 18 of pregnancy) and 16 fetuses at gestational ages of 8 and 18 days were collected. All specimens were stained for ERs using standard immunohistochemical techniques. Results: ERs are present in the cochlea of the rat and vary during maturation and pregnancy. No ERs were found in the fetal cochleas. Of the non-fetal time points measured, the expression levels of ERs in the rat cochlea were highest at the postnatal age of 21 days and were lowest during late pregnancy (day 18).

• Estrogen receptor alpha
• estrogen receptor beta
• organ of Corti
• spiral ganglion
• fetus
• outer hair cell
• hearing