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The effect of ouabain on cochlear potentials and endolymph composition was studied in guinea pigs. Introduction of ouabain into the perilymphatic space resulted in a serious depression of cochlear potentials, and a rise of sodium and fall of potassium content in the endolymph. Lowering the concentration of sodium in the perilymph minimized the action of ouabain. Some general relations between cochlear potentials, endolymph and active ion transport mechanism were discussed.
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