Histochemistry of the Normal Eustachian Tube

Abstract

Histochemical studies of various cellular components of the normal eustachian tube have been undertaken in the guinea pig, cat and squirrel monkey. Oxidative and hydrolytic enzymes were strongly active in ciliated columnar cells, less active in goblet cells, suggesting active metabolism. In general, cellular components of the subepithelial layer reacted strongly to the various enzymes. Of the glands studied, serous cells contained more oxidative enzymes than did mucous cells. Within the hyaline cartilage the chondrocytes demonstrated fairly strong enzyme reactions, the intercellular matrix none. These findings assist our understanding of normal eustachian tube physiology regarding ciliary clearance and lubrication and serve as a basis for understanding inflammatory diseases such as the middle ear effusions.