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Acta Oto-Laryngologica Volume 90, 1980 - Issue 1-6
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Original Article

Structure of the Hair Rootlets on Cochlear Sensory Cells by Tannic Acid Fixation

M. Itoh Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
&
T. Nakashima Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
Pages 385-390 | Received 24 Sep 1979, Published online: 08 Jul 2009
 
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Abstract

Rootlets of the sensory hairs of the basal turn of the guinea pig cochlea were investigated using the tannic acid fixation method under transmission electron microscopy (TEM). The fine structure of the rootlet was enhanced by adding tannic acid to the prefixative for the specimen. By using the tannic acid fixation method. it is possible to observe the finer filamentous structure of the rootlet in greater detail than is revealed by using the ordinary glutaraldehyde-osmium fixation method. TEM observation by the tannic acid method reveals that the rootlet consists of 50 å diameter protofilaments and an electrondense background substance. These protofilaments are arranged in an extremely regular pattern. They are considered to be modified microfilaments.

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Related Research Data

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