Intratympanic dexamethasone up-regulates Fkbp5 in the cochleae of mice in vivo

Abstract

Conclusions: Quantitative, real-time RT-PCR demonstrated that intratympanic dexamethasone significantly up-regulates the expression of Fkbp5 in cochleae of mice in vivo. The immunohistochemistry results showed fundamentally ubiquitous expression of Fkbp5 in cochlear structures, with relatively strong expression in type 4 fibrocytes and weak signal in the inner hair cells. These data indicate that dexamethasone regulates gene expression at the level of transcription in vivo and that this process is basically ubiquitous in the cochlea. Objectives: To demonstrate that intratympanically applied dexamethasone up-regulates Fkbp5 in the cochlea in vivo. Methods: Dexamethasone or control saline were intratympanically applied to adult C57/BL6 mice and dexamethasone-dependent changes in the levels of Fkbp5 expression in the cochlea were analyzed using quantitative real-time RT-PCR. The expression pattern of Fkbp5 in cochlea was investigated by immunohistochemistry in mice that were administered dexamethasone and in controls. Results: Quantitative real-time RT-PCR demonstrated significant increases of Fkbp5 expression levels in cochleae of dexamethasone-treated mice as compared with controls at 12 h after application (244.8 ± 155.5, n = 5 vs 100.0 ± 3.0, n = 6, p < 0.01). Immunohistochemistry showed fundamentally ubiquitous expression of Fkbp5 in cochlear structures, with some strongly positive fibrocytes in the spiral ligaments and weak immunoreactivity in the inner hair cells. Distribution of Fkbp5 signaling was not different between the dexamethasone-treated group and controls.

Keywords::

• Glucocorticoid
• gene expression
• sensorineural hearing loss
• quantitative real-time RT-PCR
• immunohistochemistry

Acknowledgments

This work was supported by a grant from Japanese Ministry of Health, Labour and Welfare (H23-NANCHI-IPPAN-021).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.