Changes in electrical response function and myosin heavy chain isoforms following denervation and reinnervation of bilateral posterior cricoarytenoid muscles in dogs

Abstract

Conclusions: Both electrical response function and mRNA expression of myosin heavy chain (MyHC) types 2X, 1, and Neonatal of bilateral posterior cricoarytenoid (PCA) muscle changed after denervation or reinnervation in canines. Objectives: There is a need to investigate the electrical response function MyHC alteration of denervation or reinnervation in the bilateral PCA muscle of large animals. Methods: MyHC isoforms expression profile and PCA muscle function outcome were detected by real-time reverse transcribed-polymerase chain reaction and muscle response to functional electrical stimulation, 9 weeks after denervation and reinnervation with ansa–recurrent laryngeal nerve anastomosis in dogs. Results: Denervation produced up-regulation of MyHC-1 and MyHC-Neonatal messenger ribonucleic acid (mRNA) expression. Reinnervation caused a decrease of MyHC-2X mRNA expression. The electrical voltage threshold of vocal fold movement and maximum abduction of denervation were greater than that of the reinnervated or control group. The denervated vocal abduction maximum of response to electrical stimulation was less than that in reinnervation or control groups.

Keywords::

• Electrical stimulation
• regeneration
• recurrent laryngeal nerve
• canine
• anastomosis
• abduction

Acknowledgments

The authors thank Liming Yao, ZhouCuo Qi, and Xianbing Wang for assistance with manuscript preparation and data interpretation. This work was supported by Guangzhou Government ZhiCheng Plan Funding (grant no. 11A56040659) and Guangdong Province Government Technology Research and Developing Funding.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.