ELISA for Determination of Immunoreactive Free Elastase and Elastase in Complex with α₁-antitrypsin in Nasal Secretions with Sinusitis

Abstract

Hamaguchi Y, Suzumura H, Taya M, Sakakura Y. ELISA for determination of immunoreactive free elastase and elastase in complex with α₁-antritrypsin in nasal secretions with sinusitis. Acta Otolaryngol (Stockh) 1991; 111: 542-549.

Sensitive double antibody sandwich ELISA methods was developed in order to quantify immunoreactive neutrophil elastase (NE) levels in nasal secretions with chronic sinusitis (CS). Microwell plate as a solid phase was coated with anti-NE antibody. Two different horseradish peroxidase (HRP) labelled antibodies used as the second antibody were anti-NE-HRP for measuring total (free+complexed) NE level and anti-α₁-antitrypsin (AT)-HRP for complexed NE level. Mean value of total NE was 31.0+20.7 ug/ml in nasal secretions from adult patients with CS, and the percentage of complexed NE in total NE was 33.7+21.4%. This sandwich ELISA is a useful method for measuring both total and complexed NE levels in nasal secretions.

Key Words:

• alpkarprotease inhibitor
• chronic sinusitis
• neutrophil elastase