Expression of Glycoconjugates in Normal and Sjögren's Syndrome Labial Glands

Abstract

Glyconjugate expression in formalin-fixed, paraffin-embbeded Sjögren's syndrome labial glands (nine cases) was compared with that in normal glands (12 samples) using a wide panel of lectins. Mucous cells expressed mainly mucous-type glycoproteins including sialyl, fucosyl, galactosyl and galactosaminosyl residues, and some N-Iinked glycoconjugates. Demilunar cells expressed mainly αD-mannose, GlcNAc and Gal β,1,3-GalNAc. Duct cells and cellular glycocalyx expressed O-linked and N-linked residues. Sjögren's syndrome samples showed a statistically significant increase in the expression of GlcNAc. A significantly decreased expression of Gal β 1,3-GalNAc and αD-mannose, residues not usually present in mucous cells, was found (p < 0.05). This suggests that anatomic rather than functional alterations determine mucous cell impairment and the symptoms in Sjögren's syndrome.

Key Words:

• lectin histochemistry
• minor salivary glands
• glycoproteins