Age-Dependent Responses of Ciliary Ganglion Neurons to Conditioned Med] on Cells at Different Stages of Embryonic Development

Abstract

Neurons from ciliary ganglia (CG) from 8 to 14 day-old chick embryos were cultured in presei of conditioned media (CM) by eye tissue cells (ETC) on nonneuronal cells from ciliary ganglia (NFGC). ese conditioning cells were obtained from 8 and 14 day-old emrbyos. Two parameters, surviving neuror and neurons displaying neurites, were determined after 48 h of culture. For neuronal survival, CMs di not show an effect on CG14 neurons. In the other neuronal ages ETC-CMs maintained a similar ne nal survival, whereas NFGC-CMs were more effective on older neurons. CM 14 media were more eff ive maintaining neuronal survival than CM8 media respectives. The number of neurons displaying ne ites decreased with neuronal ages in presence of all CMs. ETC8-CM was the better promoting neurite exteion in all neuronal ages tested.

Key Words:

• parasympathetic neurons
• cultured neurons
• neuronal survival
• neurite outgrowth
• chick enyo