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Abstract
The function of classical GABAA receptors of the rabbit Deiters' neurons has been studied at the single membrane level by a biochemical micromethod involving the study of labelled chloride permeation. In particular, labelled chloride permeation across microdissected fresh single membranes was studied in a microchamber system. The stimulation of 36Cl− out in permeation by “extracellular” GABA was determined under different conditions in the respect of Ca++. When the conditions were such that “intracellular” Ca++ was 0.02 μ there appeared to be an optimal effect by GABA on chloride passage. Conditions presumably resulting in an increase of [Ca++]i beyond the level reported above led to a decreased GABA effect, especially at the highest GABA concentrations used (± lO−4M). However, complete removal of Ca++ by a high (12mM) intracellular EGTA concentration erased completely the GABA effect.