The activity of paraoxonase and arylesterase in patients with osteomyelitis

Abstract

Background. The aim of this study was to evaluate oxidative stress and to determine the activity of paraoxonase and arylesterase in patients with osteomyelitis compared to healthy controls. Method. In total, 30 patients diagnosed with osteomyelitis and 30 healthy volunteers were enrolled in the study. Paraoxonase and arylesterase activities were measured spectrophotometrically. Serum lipid hydroperoxide (LOOH) concentrations were measured by ferrous oxidation with xylenol orange (FOX) assay as markers of oxidative stress. Results. Serum paraoxonase and arylesterase activities were significantly lower in patients with osteomyelitis compared to control individuals (all p < 0.05). Serum LOOH concentrations were significantly higher in patients with osteomyelitis than those in controls (p < 0.05). Arylesterase activity was inversely correlated with triglyceride (r =− 0.49; p = 0.005) and cholesterol concentrations (r =− 0.41; p = 0.025). Conclusion. In light of the findings obtained from the present study, it may be assumed that decreased activity of serum paraoxonase and increased concentrations of LOOH observed in osteomyelitis patients appear to be related to the increased oxidative stress and inflammatory conditions present in these patients, and may cause a much more severe status of the disease.

Key Words::

• Paraoxonase
• 1
• arylesterase
• osteomyelitis
• lipid
• hydroperoxide

Acknowledgments

We thank Assis. Prof. Dr Ibrahim Koruk from Harran University Medical Faculty Public Health Department for the the statistical analysis of the study.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of paper.

This study was supported by Harran University Scientific Research Projects Commission Grant number: 2009/1013.