A Micro Biuret Method for Protein Determination Determination of Total Protein in Cerebrospinal Fluid

Abstract

A rapid and convenient colorimetric method for the determination of small amounts of protein, based on the biuret reaction and readings at 330 mμ, is described. The “micromethod” allows the determination of 10 to 200 mg per cent protein with an acurassy of ± 1.5 mg per cent. An “ultra micro” modification allows determinations of 10 to 400 micrograms protein.

The method is applied for the determination of total protein in 1 ml of cerebrospinal fluid.