Separation of p-Acetamidophenol Metabolites by Gel Filtration on Sephadex G 10

Abstract

Jagenburg, R., Nagy, A. & Rödjer, S. Separation of p-Acetamidophenol Metabolites by Gel Filtration on Sephadex G 10. Scand. J. clin. Lab. Invest. 22, 11-16, 1968. By gel filtration on Sephadex G 10, five metabolites of p-acetamidophenol (acetaminophen) could be separated and quantitatively determined in urine. The quantitatively most important metabolites were p-acetamidophenol-glucuronide and p-acetamidophenol-sulphate. Minor quantities of S-(1-acetamido-4-hydroxyphenyl)-cysteine and free p-acetamidophenol were also found. Data are presented indicating that the fifth metabolite was 1-acetamido-4-hydroxyphenylmercapturic acid, which was also excreted in small amounts.

Key Words:

• Gel filtration
• acetaminophen