Interaction of Bromphenol Blue and Bilirubin with Bovine and Human Serum Albumin Determined by Gel Filtration

Abstract

Bjerrum, O. J. Interaction of Bromphenol Blue and Bilirubin with Bovine and Human Serum Albumin Determined by Gel Filtration. Scand. J. clin. Lab. Invest. 22, 41-48, 1968.

A gel filtration method, after Hummel & Dreyer, for determining the binding of bromphenol blue, in a 200-fold concentration range, to bovine and human serum albumin at a physiological pH, is described in detail.

The experiments show that this binding may be divided into two classes, each having its binding constant, with 3 and 4 binding sites respectively. For human serum albumin at pH 7.41 k₁ = 1.5·10⁶ and k₂ = 2.5·10⁴. By competition with bilirubin, bound to albumin before the binding experiment with bromphenol blue, it was demonstrated that bilirubin is bound by one mole in each of the two classes.

Key Words:

• Albumins
• bilirubin
• gel filtration
• sulfonphthaleins