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Abstract
An improved technique has been developed for the separation and isolation of plasma lipoproteins with zonal Ultracentrifugation. The lipoproteins of 25 ml plasma were fractionated by flotation in a MSE type B-XV titanium rotor at 120,000 X g for 18 hours using a NaCl-KBr density gradient from d = 1.005 to d = 1.23. Very low density, low density and high density lipoproteins were separated from each other and from the other plasma proteins, and the high density lipoproteins were divided into two subfractions.