Synthesis of Phospholipids and Triglycerides in Human Liver Slices I. Experimental Conditions and the Synthesis Rate in Normal Liver Tissue

Abstract

Human liver slices were incubated with glycerol-1,3-¹⁴C or fructose-U-¹⁴C. 50% of the label was recovered as glucose and 10% was incorporated into CO₂ and into lipids, respectively. At high fatty acid concentration in the medium the incorporation into CO₂ and glucose was suppressed, into triglycerides increased, and into phospholipids unchanged. The incorporation of fructose was suppressed with glycerol in the medium. The mean synthesis rate for phospholipids was 0.14 ± 0.01 μmoles/g liver per hour and for triglycerides 0.34 ± 0.04 μmoles/g liver per hour.

Key Words:

• Fructose-U-¹⁴C
• lipid synthesis
• glycerol-1,3-¹⁴C
• lipid synthesis
• glycerol metabolism
• liver
• lipid metabolism
• liver slices
• phospholipid synthesis
• liver
• triglyceride synthesis
• liver