Effect of Hog Vitamin B₁₂ Binders on the Uptake of Vitamin B₁₂ by Tissue Homogenates

Abstract

Two vitamin B₁₂ binding components were separated from the hog intrinsic concentrate WES 942 by recycling gel filtration and examined for enhancing effect on the uptake of B₁₂ by tissue homogenates. Only the minor component, B, which according to an earlier study possessed intrinsic factor identity, enhanced the uptake of B₁₂ by guinea pig intestinal mucosa homogenate. Only the major B¹² binding component, A, which had earlier been found to lack intrinsic factor identity, enhanced the uptake of B¹² by rat-liver homogenate.

Key Words:

• Immunologic identification
• intestinal mucosa
• intrinsic factor
• liver
• transport proteins
• vitamin B₁₂