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Scandinavian Journal of Clinical and Laboratory Investigation Volume 24, 1969 - Issue 4
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Original Article

Bilirubin Colloid Formation in Neutral Aqueous Solution

R. Brodersen Department of Biochemistry A, University of Copenhagen, Copenhagen, Denmark
&
J. Theilgaard Department of Biochemistry A, University of Copenhagen, Copenhagen, Denmark
Pages 395-398 | Received 25 Jun 1969, Accepted 27 Aug 1969, Published online: 08 Jul 2009
 
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Abstract

Bilirubin dissolved in sodium hydroxide solution and neutralized to pH between 7.4 and 8 changes from a genuine solution to a colloid state in the course of several hours. The colloidal solution is stable and shows a characteristic light absorption spectrum. The true solubility of bilirubin is very low, about 0.1 μm (0.005 mg/100 ml) at pH 7.4 and ionic strength 0.1 M.

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