Plasmin Digestion of Stabilized and Non-Stabilized Fibrin Illustrated by pH-Stat Titration and Thrombelastography

Abstract

SH-inhibitors and transamidase-inhibitors inhibit stabilization of fibrin in isolated systems, as defined by complete solubility in urea and in monochloroacetic acid. They also prolong k-values and reduce maximal amplitude in thrombelastography, but exhibit no significant effect on polymerization rate of fibrin as estimated by spectrophotometry. Stabilized and non-stabilized fibrin clots are digested at the same rate by plasmin according to pH-stat experiments. Presence of SH-inhibitors or transamidase-inhibitors enhance plasmin digestion in thrombelastographic experiments.

Key Words:

• Non-stabilized/stabilized fibrin
• pH-stat-titration
• plasmin digestion
• thrombelastography