A Sub-Microliter Sampling Device for Quantitative Collection of Gases. Application to ‘Gas Chromatographic’ Analysis

Abstract

For CO calibration purposes, samples—fractions of μl amounts—were taken from streaming CO, and trapped between water surfaces in the needle of a (Hamilton) microliter syringe, transferred to a ‘gas chromatographic’ system for analysis. The sampling technique was easy to perform with the aid of a slightly modified 10 μl syringe, adapted to an original ‘Agla’ burette by means of a coil spring, and a slide guided by a cylinder with a longitudinal slit. The sampling procedure is described. The calibration showed a coefficient of variation of about 2 and a good linearity (r=0.9989) for values between 0.091 and 0.546 μl CO.

Key Words:

• Calibration
• CO
• COHb
• gas chromatography
• microliter syringe
• micrometer
• sampling device
• sub-microliter sample of gas