Thin Layer Electrofocusing Followed by Electrophoresis in Antibody Containing Gel

Abstract

After thin layer electrofocusing in polyacrylamide gel electrophoresis was performed in antibody containing polyacrylamide agarose gel. The technique combines the high resolution of polyacrylamide gel electrofocusing with the high sensitivity of immunologic identification that gives semiquantitative information. The technique is demonstrated on multiple molecular forms of salivary amylase.

Key Words:

• Amylase
• Antibody containing gel
• Electrofocusing
• Polyacryl-amid gel