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Abstract
The principle used by Keitt (J. Lab. clin. Med. 77, 470, 1971) for the determination of 2,3-diphosphoglycerate has been adapted to an Auto Analyzer system in which the consumption of NADH in the glyceraldehyde phosphate dehydrogenase reaction is measured in a fluorometer. Perchloric acid extracts of blood are used and the method is applied to venous as well as to capillary blood. The coefficient of variation is 2–5%, the recovery of added 2,3-diphosphoglycerate around 95%. In 92 healthy men, 50 years of age, the concentration of 2,3-diphosphoglycerate was 0.76 ± 0.1 μmoles/mole hemoglobin (4 Fe).