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Scandinavian Journal of Clinical and Laboratory Investigation Volume 34, 1974 - Issue 4
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Original Article

Polarographic Determination of Total Oxygen Content in Small Blood Samples

L. Borgström Brain Research Laboratory, E-blocket, and Dept. of Clinical Physiology, University Hospital, Lund, Sweden
,
M. Hägerdal Brain Research Laboratory, E-blocket, and Dept. of Clinical Physiology, University Hospital, Lund, Sweden
,
L. Lewis Brain Research Laboratory, E-blocket, and Dept. of Clinical Physiology, University Hospital, Lund, Sweden
&
U. Pontén Brain Research Laboratory, E-blocket, and Dept. of Clinical Physiology, University Hospital, Lund, Sweden
Pages 375-380 | Received 06 Apr 1974, Accepted 10 Jul 1974, Published online: 08 Jul 2009
 
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Abstract

The performance of a method for determination of oxygen content in 25-μl blood samples, using commercially available equipment, is described. The method gives a coefficient of variation in total oxygen content of 1 to 2 per cent and allows measurements of 10 to 12 samples within an hour. Good agreement was obtained with results obtained from titrations of hydrogen peroxide. There were no systematic differences between the To2 values obtained with the present method and those calculated from spectrophotometric determinations of oxygen saturation and hemoglobin concentration.

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