Studies on the pre-α-lipoprotein of human serum II. Evidence for the presence of an albumin-Apo-A-I complex

Abstract

By combined column affinity chromatography and preparative electrophoresis, a lipoprotein was isolated from the electrophoretically defined pre-α-region. The isolated fraction, designated Fraction II₁demonstrated one band after electrophoresis in agarose, in polyacrylamide, and in sodium dodecylsulphate containing polyacrylamid. Double diffusion experiments disclosed the presence of albumin and apolipoprotein-A-I within the fraction. After reduction with mercaptoethanol and subsequent electrophoresis in sodium dodecylsulphate containing polyacrylamide gel, two bands appeared. One of the bands had an electrophoretic mobility similar to albumin monomer (mol.wt 67,000), the other had the same electrophoretic mobility as apolipoprotein-A-I with a mol.wt of 28,400. It is suggested that Fraction II₁ contains an albumin-apolipoprotein-A-I complex.

Key Words:

• apolipoprotein-A-I
• immunodiffusion
• immunization
• polyacrylamide gel electrophoresis