Enzymatic determination of triglycerides with a System Olli 3000 analyser

Abstract

We describe a totally enzymatic method for determining serum triglycerides Actapted for a System Olli 3000 analyser. In this procedure triglycerides are determined by measuring free glycerol enzymatically after hydrolysis of a sample with lipase and esterase. The method utilizes two standards for calibration, includes a blank correction and requires 20 μ1 of serum. Forty serum samples can be analysed in about 30 min. The procedure is linear up to a concentration of 8 mmol/1 of triglycerides. The precision and sensitivity of the method are good. A comparison of this method with another enzymatic method gave the correlation coefficient 0.988 and the regression line y = 1.02x + 0.02 (n2 = 95).

Key Words:

• automation
• glycerol, determination of
• triglycerides, determination of