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Original Article

Determination of alkaline phosphatase (EC 3.1.3.1) on the SMAC using diethanolamine in a modified manifold

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Pages 279-281 | Received 22 Jun 1978, Accepted 13 Nov 1978, Published online: 08 Jul 2009
 

Abstract

The SMAC method for alkaline phosphatase was modified inorder to determine the enzyme activity according to the Scandinavian recommended method [1]. The method is not strictly ‘Scandinavian’, but the correlation with the strict Scandinavian method performed on the LKB 8600 Reaction rate analyzer was excellent. The modified method was linear up to at least 2000 U/l.

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