The kynurenine load test, an adjunct to the tryptophan load test

Abstract

The kynurenine load test was introduced to distinguish, in vivo, between abnormal tryptophan metabolism caused by induction of tryptophan oxygenase or by impaired kynureninase function. This test was carried out by giving a loading dose of 700 μmol L-kynurenine sulphate followed by determination of urinary metabolites of the tryptophan-nicotinic acid ribonucleotide pathway. The results of the kynurenine load test in a group of healthy post-menopausal female subjects show a consistant increase in urinary excretion of some metabolites of the tryptophan-nicotinic acid ribonucleotide pathway (kynurenine, 3-hydroxy-kynurenine, and 3-hydroxyanthranilic acid) of a magnitude comparable to the increase seen after a loading dose of 9800 μmol L-tryptophan (the tryptophan load test).

Key Words:

• hydrocortisone
• kynurenine
• tryptophan
• vitamin B₆