Detection of hyperlipidemia by semiquantitative fluorometric determinations of serum lipids on thin-layer chromatograms

Abstract

In one analytical procedure, semiquantitative information concerning the concentrations of triglycerides, free and esterified cholesterol were obtained. Twenty-five microlitres of serum were used and the concentrations were classified as high, borderline or normal compared to a lipid standard solution. Thirty-four patients were investigated and the results showed good agreement with the results from conventional screening for hyperlipidemia by separate quantitative analyses of total cholesterol and triglycerides.

Key Words:

• cholesterol esters
• free cholesterol
• screening
• thin-layer chromatography
• triglycerides