Haemoglobin determination without cyanide on Hemalog-8

Abstract

The most common method for determination of haemoglobin is the cyanmet-haemoglobin method which is recommended by the International Committee for Standardization in Haematology (ICSH). Determination of haemoglobin is one of the most used methods in hospital laboratories. One disadvantage with the method is the use of cyanide in the reagent, which demands cautious preparation and use. The intention of our investigation was to replace the cyanide reagent for Hemalog-8 with a reagent not containing cyanide. It is shown that the change of reagent did not influence the results of the analysis.

Key Words:

• carbonmonoxide haemoglobin
• cyanmethaemoglobin
• haematology
• oxyhaemoglobin