Rapid quantitative determination of C-reactive protein using laser-nephelometer

Abstract

A rapid quantitative immunonephelometric method to determine C-reactive protein is described. Quantitative results are obtained after 1 h incubation. The precision of the method is good and the sensitivity is approximately the same as the sensitivity of the immunodiffusion plates, which are commercially available. A comparison of the nephelometric method with the radial immunodiffusion method gave the correlation coefficient 0.968 and the regression line y = 1.111x -0.005 (n = 85).

Key Words:

• acute phase proteins
• light scattering
• nephelometry
• proteins
• quantitative determination of