Immunoassay for transferrin using LKB 8600 Reaction Rate Analyzer

Abstract

A rapid immunochemical method for transferrin determination is described. The method is based on measuring turbidity caused by antigen-antibody complexes with an enzyme analyser using diluted antiserum as a starting reagent. This immunoturbidimetric method correlates well with TIBC (r=0.979; n=36). A comparison with the radial immunodiffusion method gave the correlation coefficient 0.984 and the regression line y=1. 20x-0.54 (n=36).

Key Words:

• immunoturbidimetry
• proteins
• quantitative determination of
• total iron-binding capacity