An improved specific and sensitive radioenzymatic method for determination of serotonin concentrations in biological fluids (radioenzymatic serotonin method)

Abstract

Existing radioenzymatic assays for determining the concentration of serotonin in biological fluids have been further modified resulting in increased sensitivity and a lowered detection limit (0.017 pmol). The assay has been used for determinations in platelet-poor plasma (3.3-20.5 nmol/l) and cerebrospinal fluid (0.15-20 nmol/1) from man.

Key Words:

• blood platelets
• cerebrospinal fluid
• 5-HT
• N-acetylserotonin
• platelet-free plasma
• S-adenosyl-L-(³H-methyl)-methionine