Preparation of frozen reference sera and examples of applications

Abstract

We describe procedures for preparation of human serum from ACD bank blood and bovine serum from slaughter house blood. Preparation techniques including filtration and dialysis are described in detail as well as how to obtain desired concentrations. Special attention has been drawn to procedures yielding low lactate dehydrogenase activity. Long-term storage should take place at -75°C. Different frozen reference sera have been used for calibration purposes, internal quality control and external quality assessment.

Key Words:

• bovine sera
• calibration sera
• external quality assessment
• internal quality control
• lactate dehydrogenase
• stability
• storage
• turbidity