A method for determination of immunoreactive trypsin in complex with α₁-antitrypsin in human sera

Abstract

A double antibody solid phase radioimmunoassay for the determination of cationic trypsin in complex with a,-antitrypsin in human sera is described.

No immunoreactive trypsin in complex with α₁-antitrypsin can be detected in normal sera. In sera from 8 patients with acute pancreatitis levels between 100 and 1000 μg/l are seen. The amount of trypsin in complex with α₁-antitrypsin in serum in acute pancreatitis equals or exceeds the amount of trypsinogen. Serum levels of trypsin in complex with α₁-antitrypsin also remain elevated longer than the trypsinogen levels in acute pancreatitis.

Key Words:

• α₁-antitrypsin
• acute pancreatitis
• trypsin