Assay of intestinal disaccharidases

Abstract

A modified and updated description of the author's method for the assay of intestinal disaccharidases. The principle of the method is the following: an intestinal homogenate is incubated with the appropriate disaccharide. The disaccharidase activity is then interrupted by the addition of TRIS, and the glucose liberated is measured with a glucose oxidase reagent.

Key Words:

• disaccharidases
• assay method
• disaccharide intolerance
• lactose intolerance
• disaccharidase deficiency
• α-glucosidases
• β-galactosidases
• sucrase
• maltase
• isomaltase
• trehalase
• lactase