Determination of Gamma Glutamyltransferase in Completely Haemolysed Blood Samples

Abstract

Gjerde H, MØrland J. Determination of gamma glutamyltransferase in completely haemolysed blood samples. Scand J Clin Lab Invest 1985; 45: 661–664.

A simple method for the determination of gamma glutamyltransferase (GGT) in completely haemolysed blood samples is described. Haemolysed blood was incubated in the presence of gamma glutamyl-p-nitroanilide and glycylglycine at 37 d`C for 15 min, and the enzyme reaction was terminated by adding trichloroacetic acid. The pH in the supernatant was adjusted to 7.5 by using a buffer, and the amount of p-nitroaniline formed was measured spectrophoto-metrically. The correlation between this method and the GGT activity in serum as determined by the standard kinetic method was good, r=0.982. The blood GGT activity (y) was related to the serum activity (x) as follows: y=0.415x + 3.8. The effect of storage of blood at room temperature on the GGT activity was studied. The enzyme activity did not change during the first 12 days of storage.

Key Words:

• blood
• gamma glutamyltransferase
• haemolysis
• method