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Scandinavian Journal of Clinical and Laboratory Investigation Volume 45, 1985 - Issue 8
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Original Article

A simple method for evaluating platelet superoxide dismutase

F. Violi Institute of Clinical Medicine I, University of Rome, Italy
,
L. Iuliano Institute of Clinical Medicine I, University of Rome, Italy
,
C. Alessandri Institute of Clinical Medicine I, University of Rome, Italy
,
A. Ghiselli Institute of Clinical Medicine I, University of Rome, Italy
&
F. Balsano Institute of Clinical Medicine I, University of Rome, Italy
Pages 713-716 | Received 02 Jan 1985, Accepted 15 May 1985, Published online: 08 Jul 2009
 
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Abstract

Violi F, Iuliano L, Alessandri C, Ghiselli A, Balsano F. A simple method for evaluating platelet superoxide dismutase. Scand J Clin Lab Invest 1985; 45: 713–716.

A simple and rapid spectrophotometric method for evaluating platelet superoxide dismutase is reported. Platelets prepared avoiding erythrocyte and leucocyte contamination were lysated and tested in a Tris-cacodylic buffer containing pyrogallol. Platelet superoxide dismutase was calculated by evaluating the degree of the pyrogallol autoxidation inhibition induced by platelet lysate. Possible interferences of hydrogen peroxide, peroxidases and reducing substances were excluded. Platelet superoxide dismutase content was studied in 38 healthy subjects and was 19.1±4.1 U/108 platelets or 35±7.8 U/mg protein.

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