Effects of insulin on the total number of ³H] ouabain binding sites in normal human ymphocytes and after stimulation in vitro with concanavalin A

Abstract

The effects of insulin in vitro on the total number of [³H]ouabain binding sites were studied in normal and concanavalin A-stimulated human T-lymphocytes after incubation for 18 and 42 h with 0, 10² and 10⁵ mIU/l of insulin. While no effect on [³H]ouabain binding could be demonstrated in non-stimulated cells, a significant decrease could be produced in concanavalin A-stimulated cells. Mean±SD for the total number of [³H]ouabain binding sites per cell after 18 h incubation time with concanavalin A in the absence of insulin was 46,099±6,620 as compared with 44,783±8,347 in the presence of 10² mIU/l of insulin (nonsignificant) and 42,406±7,066 in the presence of 10⁵ mIU/1 (p=0.031). The corresponding 42-h values were 47,075±9,412, 43,761 ±9,273 (p=0.033) and 43,824±9,312 (p=0.005).

Key Words:

• glycocide receptors
• insulin receptors
• lectines
• Na⁺/K⁺-ATPase, type 2 diabetes mellitus