Effect of freezing and ultrasonication on the density of human lymphocyte Adrenoceptors

Abstract

In this study 21 pregnant volunteers were divided into three equal groups; blood samples were taken from each, and after isolation of the lymphocytes the cell suspensions were divided into two equal samples. The density of β₂-adrenoceptor in intact lymphocytes was compared with the receptor density for lymphocytes stored 7 days or 19 days at -70 ^oC and with cells disrupted by ultrasonication. Storage by lymphocytes at -70 ^oC for 7 days to 19 days decreased the β₂-adrenoceptor density by 20% and 37%, respectively; ultrasonication decreased the density by 65%.

Key Words:

• beta adrenergic receptor
• cyclic AMP receptors
• lymphocytes