Superoxide release by primary-cultured human hepatic macrophages and peripheral monocytes from patients with normal and cirrhotic livers

Abstract

We analysed superoxide anion (O₂⁻) release from primary-cultured human hepatic macrophages (HHMΦ) and peripheral blood monocytes (MO) derived from patients with normal and cirrhotic livers. Primary cultured human hepatic macrophages and MO from cirrhotic patients released less O₂⁻ than cells from normal patients. Superoxide anion release by HHMΦ showed a significant, positive correlation with the serum glutamate pyruvate transaminase (GPT) level, whereas O₂⁻ release by MO showed only a weak correlation with the GPT level. In conclusion, a significant reduction in O₂⁻ release was observed in HHMΦ and MO cultured from cirrhotic patients. Primary-cultured human hepatic macrophages are probably more susceptible to environmental changes within the cirrhotic liver than MO, since they are found locally within the liver and correlate with serum GPT levels.

Key Words:

• human hepatic macrophage
• monocyte
• superoxide
• liver cirrhosis