Luminol enhanced Fc-receptor dependent chemiluminescence from peripheral PMN cells. A methodological study

Abstract

Maximal luminol enhanced chemiluminescence (CL) of PMN cells after Fc-gamma-receptor stimulation is a way to study the cell activity in connection with phagocytic function. Optimal conditions for such a method were elaborated for practical clinical use. After blood sampling and gentle mixing the blood sample was allowed to stand at +20 ^oC for not more than 1 h. The PMN cells prepared at +20 ^oC were washed at +4^oC with a phosphate buffer containing human serum albumin. A gentle lysis of the red blood cells with NH₄Cl solution reduced the number of red cells sufficiently not to interfere with the CL. Important factors for the precision of the method were reproducible amounts of bacteria and a reproducible mixing of the particles during the CL analysis. The method had a variation (CV) of 10-15% in healthy individuals.

Key Words:

• PMN cell function
• Fc-receptors
• chemiluminescence
• luminol