Oral S-adenosyl-L-methionine (SAMe) administration enhances bile salt conjugation with taurine in patients with liver cirrhosis

Abstract

We investigated whether the oral administration of SAMe influences the hepatic availability of sulphur amino acids and the extent of bile salt amidation with taurine in liver cirrhosis. Ten patients with cirrhosis (eight Child-Pugh A and 2 B, aged 48-65 years), were studied before and 2 months after oral SAMe administration (800 mg per day). Bile was obtained using a string-test device (Entero-test), after gall-bladder contraction with caerulein. No significant changes were found in the per cent composition of biliary amino acids, except for an increase in glutamic -acid (from 3.7 ± 0.6% before to 6.1 ± 1.1% after SAMe, p = 0.003) and taurine from 2.2 ± 2.3% (range 0.4-6.8) to 7.2 ± 9.2% (range 0.5-28.1), (NS). HPLC analysis showed a trend towards increased per cent tauroconjugation of all individual bile salts, with a significant rise in tauro-chenodeoxycholic acid (from 15.0 ± 9.4% to 25.3 ± 9.7%, p = 0.05) and a drop in glycocholic acid (from 39.1 ± 15.3% to 25.3 ± 9.8%, p = 0.05). These data suggest that in the cirrhotic liver exogenous SAMe is partially metabolized to taurine, which is used for bile salt amidation.

Key Words:

• tauroconjugation
• transsulfuration